Waters Xevo G2-S QToF

Xevo® G2-S QTof incorporates StepWave™ ion optics for unsurpassed levels of durable sensitivity and proven quantitative time-of-flight (QuanTof™) technology to deliver superior UPLC®-compatible mass resolution, matrix-tolerant dynamic range, quantitative performance, mass accuracy, and speed of analysis – simultaneously. Xevo G2-S QTof delivers not only conventional MS and MS/MS methods of data acquisition, it also has the ability to perform UPLC®/MSEto collect the maximum amount of data from a single analysis. UPLC/MSE datasets provide a comprehensive digital record of your sample. The system incorporates IntelliStart™ Technology, for auto-mated system optimization and status monitoring, ensuring that the highest quality data is routinely available to all levels of operator.

Acquisition modes
MS scanningMS/MS product ion scanning UPLC/FastDDA (rapid, automated MS to MS/MS scan function switching):UPLC/MSEIonization mode switching (ESCi) External contact start/stop/eventsAnalogue channel acquisition via an e-SAT/IN module

Mass range
The TOF mass range is m/z 20 to 100,000 The quadrupole mass range is m/z 20 to 16,000 in non-resolving mode and m/z 20 to 4,000 in resolving mode A high mass quadrupole option is available with a mass range up to m/z 100,000in non-resolving mode and m/z 32,000 in resolving mode

Mass measurement accuracy
The mass measurement accuracy of the instrument, in resolution mode, will be better than1 ppm RMS, based on 10 consecutive repeat measurements of the + ion of raffinose(m/z 527.1588), using a suitable choice of lock mass.

Dynamic range
The dynamic range, defined as the range of peak intensities that will give better than 3 ppm RMS for 10 sec of data, is at least four orders of magnitude when measured on the m/z 556.2771 peak from leucine enkephalin. This can be increased with use of programmable dynamic range enhancement (pDRE) technology.

Mass resolution
Resolution Mode: >32,500 FWHM measured on the (M + 6H)6+ isotope cluster from bovine insulin (m/z 956) at a data acquisition rate of 30 spectra per second.

Sensitivity Mode: >22,500 FWHM measured on the (M + 6H)6+ isotope cluster from bovine insulin (m/z 956) at a data acquisition rate of 30 spectra per second.

MS sensitivity (ESI+)
The peak at m/z 556 from a solution of 50 pg/μL leucine enkephalin in 50/50 acetonitrile/water + 0.1% formic acid, will have an intensity of greater than 6,400 counts per sec. The instrument will be tuned to >32,500 FWHM resolution (as demonstrated on bovine insulin) and the mass range will be set to m/z 1200.

MS sensitivity (ESI-)
The peak at m/z 503 from a solution of 500 pg/μL raffinose in 70/30 acetonitrile/water (no additives), will have an intensity of greater than 7,200 counts per second. The instrument will be tuned to >32,500 FWHM resolution (as demonstrated on bovine insulin), and the mass range will be set to m/z 1200.

MS/MS sensitivity
Using a -Fibrinopeptide B solution of 100 fmol/μL with the instrument tuned for 32,500resolution (as demonstrated on bovine insulin), the intensity of the most intense y sequence ion from the MS/MS spectrum of the doubly charged precursor ion (785.8 Da) will be greater than 500 counts per second.